control mab 4h2 Search Results


fibronectin  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank fibronectin
( a – c ) NCs undergo deacetylation in vivo . ( a ) Schematic showing the plane of sectioning. ( b ) In the upper panel, representative confocal projections of transverse cryosections showing highlighted NCs nuclei (cyan) and <t>fibronectin</t> (magenta) at non-migratory and migratory stages; in the lower panel, colour-coded projections of the acetylated α-Tubulin channel are shown (Scale bar 100 μm); an inset from the NCs region emphasising the signal differences between both stages is shown in the upper right corner (Scale bar 50 μm). ( c ) Normalized ratio of acetylated α-Tubulin/α-Tubulin fluorescence intensities; spread of data from the indicated conditions, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney ****P<0.0001, CI= 95%, n non-migratory = 17, n migratory = 17 embryos. ( d – i ) Graft experiments. ( d,f ) wildtype stage 17.5 (pre-migratory) NCs grafted into wildtype host embryos. ( e,g ) Hyperacetylated stage 17.5 NCs grafted into stage 17.5 wildtype host embryos (Scale bar for f and g 200 μm). ( h ) Percentage of embryos displaying NC migration; histograms represent media, error bars SD. ( i ) Normalised displacement of NCs along the dorso-ventral axis; red lines represent media, whiskers standard deviation (SD) (two-tailed t-test, ****P<0.0001, CI= 95. In f and g , n control = 22 animals, n hyperacetylated = 22 animals). ( j,k ) In vivo atomic force microscopy ( i AFM) measurements. ( j ) Diagram showing the regions measured. ( k ) Spread of data for each condition stated in the figure, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney test, ****P<0.0001, ***P<0.0009, CI= 95%, n controlNC St13 = 13, n ControlNC St21 = 10, n hypercatilated NCs St21 = 12; n hypoacetilated NCs St21 : 10 embryos). Panels in b , f , g are representative examples of at least 3 independent experiments.
Fibronectin, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fibronectin/product/Developmental Studies Hybridoma Bank
Average 96 stars, based on 1 article reviews
fibronectin - by Bioz Stars, 2026-04
96/100 stars
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anti- mouse pd- 1 mab (clone 4h2)  (Ono Pharma)
90
Ono Pharma anti- mouse pd- 1 mab (clone 4h2)
( a – c ) NCs undergo deacetylation in vivo . ( a ) Schematic showing the plane of sectioning. ( b ) In the upper panel, representative confocal projections of transverse cryosections showing highlighted NCs nuclei (cyan) and <t>fibronectin</t> (magenta) at non-migratory and migratory stages; in the lower panel, colour-coded projections of the acetylated α-Tubulin channel are shown (Scale bar 100 μm); an inset from the NCs region emphasising the signal differences between both stages is shown in the upper right corner (Scale bar 50 μm). ( c ) Normalized ratio of acetylated α-Tubulin/α-Tubulin fluorescence intensities; spread of data from the indicated conditions, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney ****P<0.0001, CI= 95%, n non-migratory = 17, n migratory = 17 embryos. ( d – i ) Graft experiments. ( d,f ) wildtype stage 17.5 (pre-migratory) NCs grafted into wildtype host embryos. ( e,g ) Hyperacetylated stage 17.5 NCs grafted into stage 17.5 wildtype host embryos (Scale bar for f and g 200 μm). ( h ) Percentage of embryos displaying NC migration; histograms represent media, error bars SD. ( i ) Normalised displacement of NCs along the dorso-ventral axis; red lines represent media, whiskers standard deviation (SD) (two-tailed t-test, ****P<0.0001, CI= 95. In f and g , n control = 22 animals, n hyperacetylated = 22 animals). ( j,k ) In vivo atomic force microscopy ( i AFM) measurements. ( j ) Diagram showing the regions measured. ( k ) Spread of data for each condition stated in the figure, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney test, ****P<0.0001, ***P<0.0009, CI= 95%, n controlNC St13 = 13, n ControlNC St21 = 10, n hypercatilated NCs St21 = 12; n hypoacetilated NCs St21 : 10 embryos). Panels in b , f , g are representative examples of at least 3 independent experiments.
Anti Mouse Pd 1 Mab (Clone 4h2), supplied by Ono Pharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti- mouse pd- 1 mab (clone 4h2)/product/Ono Pharma
Average 90 stars, based on 1 article reviews
anti- mouse pd- 1 mab (clone 4h2) - by Bioz Stars, 2026-04
90/100 stars
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Image Search Results


( a – c ) NCs undergo deacetylation in vivo . ( a ) Schematic showing the plane of sectioning. ( b ) In the upper panel, representative confocal projections of transverse cryosections showing highlighted NCs nuclei (cyan) and fibronectin (magenta) at non-migratory and migratory stages; in the lower panel, colour-coded projections of the acetylated α-Tubulin channel are shown (Scale bar 100 μm); an inset from the NCs region emphasising the signal differences between both stages is shown in the upper right corner (Scale bar 50 μm). ( c ) Normalized ratio of acetylated α-Tubulin/α-Tubulin fluorescence intensities; spread of data from the indicated conditions, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney ****P<0.0001, CI= 95%, n non-migratory = 17, n migratory = 17 embryos. ( d – i ) Graft experiments. ( d,f ) wildtype stage 17.5 (pre-migratory) NCs grafted into wildtype host embryos. ( e,g ) Hyperacetylated stage 17.5 NCs grafted into stage 17.5 wildtype host embryos (Scale bar for f and g 200 μm). ( h ) Percentage of embryos displaying NC migration; histograms represent media, error bars SD. ( i ) Normalised displacement of NCs along the dorso-ventral axis; red lines represent media, whiskers standard deviation (SD) (two-tailed t-test, ****P<0.0001, CI= 95. In f and g , n control = 22 animals, n hyperacetylated = 22 animals). ( j,k ) In vivo atomic force microscopy ( i AFM) measurements. ( j ) Diagram showing the regions measured. ( k ) Spread of data for each condition stated in the figure, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney test, ****P<0.0001, ***P<0.0009, CI= 95%, n controlNC St13 = 13, n ControlNC St21 = 10, n hypercatilated NCs St21 = 12; n hypoacetilated NCs St21 : 10 embryos). Panels in b , f , g are representative examples of at least 3 independent experiments.

Journal: bioRxiv

Article Title: Microtubule deacetylation reduces cell stiffness to allow the onset of collective cell migration in vivo

doi: 10.1101/2021.08.12.456059

Figure Lengend Snippet: ( a – c ) NCs undergo deacetylation in vivo . ( a ) Schematic showing the plane of sectioning. ( b ) In the upper panel, representative confocal projections of transverse cryosections showing highlighted NCs nuclei (cyan) and fibronectin (magenta) at non-migratory and migratory stages; in the lower panel, colour-coded projections of the acetylated α-Tubulin channel are shown (Scale bar 100 μm); an inset from the NCs region emphasising the signal differences between both stages is shown in the upper right corner (Scale bar 50 μm). ( c ) Normalized ratio of acetylated α-Tubulin/α-Tubulin fluorescence intensities; spread of data from the indicated conditions, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney ****P<0.0001, CI= 95%, n non-migratory = 17, n migratory = 17 embryos. ( d – i ) Graft experiments. ( d,f ) wildtype stage 17.5 (pre-migratory) NCs grafted into wildtype host embryos. ( e,g ) Hyperacetylated stage 17.5 NCs grafted into stage 17.5 wildtype host embryos (Scale bar for f and g 200 μm). ( h ) Percentage of embryos displaying NC migration; histograms represent media, error bars SD. ( i ) Normalised displacement of NCs along the dorso-ventral axis; red lines represent media, whiskers standard deviation (SD) (two-tailed t-test, ****P<0.0001, CI= 95. In f and g , n control = 22 animals, n hyperacetylated = 22 animals). ( j,k ) In vivo atomic force microscopy ( i AFM) measurements. ( j ) Diagram showing the regions measured. ( k ) Spread of data for each condition stated in the figure, red lines represent median, whiskers interquartile range (two-tailed Mann–Whitney test, ****P<0.0001, ***P<0.0009, CI= 95%, n controlNC St13 = 13, n ControlNC St21 = 10, n hypercatilated NCs St21 = 12; n hypoacetilated NCs St21 : 10 embryos). Panels in b , f , g are representative examples of at least 3 independent experiments.

Article Snippet: Fibronectin (mAb 4H2 anti-FN, DSHB) and acetylated α-Tubulin (T6793, Sigma Aldrich) were used for immunostaining in histological cuts.

Techniques: In Vivo, Fluorescence, Two Tailed Test, MANN-WHITNEY, Migration, Standard Deviation, Control, Microscopy